Evaluation of antioxidant potential and bioactive metabolites of Nannochloropsis sp.

Deepa P K



Marine microalgae are a photosynthetic organism which can produce diverse metabolites, biomass and O2. The bioactive compounds produced by microalgae includes polysaccharides, polyunsaturated fatty acids, polyphenolic compounds, antioxidants, peptides, essential vitamins and minerals. The present study aimed to evaluate the antioxidant activity and bioactive metabolites of the marine microalgae Nannochloropsis sp. Various solvent (petroleum ether, aqueous, isopropanol, methanol and ethyl acetate) extract of Nannochloropsis sp. were evaluated for antioxidant activity. The ethyl acetate extract contributes maximum total antioxidant activity of 71.35 ± 0.04 mg of AAE/g of extract. The ethyl acetate extract exhibited highest on nitric oxide scavenging metal chelating and reducing power assay with % inhibition of 79.57 ± 0.215, 80.73±0.105, 0.64±0.001 at 1mg/concentration respectively. Methanolic extract showed hydroxyl radical scavenging activity of 78.72±0.21 % inhibition at a concentration of 1mg/ml. The ethyl acetate extract with highest antioxidant activity was analyzed for bioactive metabolites by GCMS. Among the 15 compounds obtained through GCMS, the major component n-Hexadecanoic acid was reported to have antioxidant activity. The results from the different types of extracts clearly indicated that the bioactive components present in the microalgae contribute significantly to the antioxidant capacity of microalgae.


Nannochloropsis, antioxidant ,GCMS

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Alugoju Phaniendra, Dinesh Babu Jestadi, Latha Periyasamy, Free Radicals: properties, sources, targets, and their implication in various diseases, IJBC, 30(1),11–26, 2015. http://doi.org/10.1007/s12291-014-0446-0

Bayani Uttara, Ajay V Singh, R.T. Mahajan, Oxidative stress and neurodegenerative diseases: A review of upstream and downstream antioxidant therapeutic options, Curr Neuropharmacol., 7(1), 65-74, 2009. https://dx.doi.org/10.2174%2F157015909787602823

Hamed Safafar, Jonathan van Wagenen, Per Moller, Charlotte Jacobsen, Carotenoids, phenolic compounds and tocopherols contribute to the antioxidative properties of some microalgae species grown on industrial wastewater, Mar. Drugs, 13, 7339–7356, 2015. Doi:10.3390/md13127069.

M.A. Prietoab, M.A.Muradob, J.A.Vázquezb, Thomas P. Currana, Analytical criteria to quantify and compare the antioxidant and pro-oxidant capacity in competition assays: The bell protection function, Food Res Int, 60, 48-58, 2014. https://doi.org/10.1016/j.foodres.2013.12.037

K. Goiris, K. Muylaert, I. Fraeye, I. Foubert, J. De Brabanter, L. De Cooman, Antioxidant potential of microalgae in relation to their phenolic and carotenoid content, J. Appl. Phycol, 24(6):1477-1486, 2012. DOI 10.1007/s10811-012-9804-6

Su-Hua Goh, M.D. Fatimah, Yusoff, Su-Peng Loh, A comparison of the antioxidant properties and total phenolic content in a diatom, Chaetoceros sp. and a green microalga, Nannochloropsis sp., J. Agric. Sci. 2(3),123-130, 2010. https://doi.org/10.5539/jas.v2n3p123

P. Prieto, M. Pineda, M.Aguilar, Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E, Anal. Biochem, 269, 337-341, 1999. https://doi.org/10.1006/abio.1999.4019

S.M. Klein, G. Cohen, A. Cederbaum, Production of formaldehyde during metabolism of dimethyl sulfoxide by hydroxyl radical generating systems, Biochemistry, 20, 6006-6012, 1981. https://doi.org/10.1021/bi00524a013

T.C.P. Dinis, V.M.C. Madeira, L.M. Almeida, Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers, Arch Biochem Biophys, 315(1):161 – 169, 1994. https://doi.org/10.1006/abbi.1994.1485

L.C. Green, D.A. Wagner, J. Glogowski, P.L. Skipper, J.S. Wishnok, S.R. Tannenbaum, Analysis of nitrate, nitrite, and nitrate in biological fluids, Anal. Biochem,126: 131-138, 1982. https://doi.org/10.1016/0003-2697(82)90118-x

M. Oyaizu, Studies on products of browning reaction: Antioxidative activities of products of browning reaction prepared from glucosamine, The Japanese journal of nutrition and dietetics, 44: 307-315, 1986. http://dx.doi.org/10.5264/eiyogakuzashi.44.307

I.D. Chkhikvishvili, Z.M. Ramazanov, Phenolic substances of brown algae and their antioxidant activity, Appl Biochem Biotechnol, 36, 289–291, 2000. Doi: 10.1007/BF02742582

V. Sasikumar, P. Kalaisezhiyen, Evaluation of free radical scavenging activity of various leaf extracts from Kedrostis foetidissima (Jacq.) Cogn., Biochemistry & Analytical Biochemistry, 3(2), 150, 2014. DOI: 10.4172/2161-1009.1000150

Gini C. Kuriakose, Muraleedhara G. Kurup, Antioxidant and hepatoprotective activity of Aphanizomenon flos-aquae Linn against paracetamol intoxication in rats, Indian J. Exp. Biol, 48, 1123-1130, 2010.

P. Sangeetha, V. Anuradha, V. Suganya, P. Bhuvana, In vitro antioxidant and radical scavenging activity of marine microalga Nannochloropsis oculate, WJPPS, 7(4), 923-937, 2018.

B. Halliwell, Oxidants and human disease: some new concepts, FASEB J, 1(5):358–364, 1987.

X. Yang , F. Yan, S. Huang , C. Fu, Antioxidant activities of fractions from long a pericarp, Food Sci. Technol. 34(2):341-345, 2014. http://dx.doi.org/10.1590/S0101-20612014005000034

Lamia Trabelsi, Olfa Chaieb, Amira Mnari, Salwa Abid-Essaf, Lotfi Aleya, Trabelsi, Partial characterization and antioxidant and antiproliferative activities of the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp., BMC COMPLEM ALTERN M, 16, 210-235, 2016. Doi: https://dx.doi.org/10.1186%2Fs12906-016-1198-6

T.M. Dawson, V.L. Dawson, S.H. Snyder, A novel neuronal messenger molecule in brain: the free radical, nitric oxide, Ann Neurol, 32: 297–311, 1992. https://doi.org/10.1002/ana.410320302

Huie RE, Padmaja S. The reaction of NO with superoxide. Free Radic. Res. Commun, 18(4), 195–199, 1993.

C. Saranya, A. Hemalatha, C. Parthiban, P. Anantharaman, Evaluation of antioxidant properties, total phenolic and carotenoid content of Chaetoceros calcitrans, Chlorella salina and Isochrysis galbana, IJCMAS, 3(8): 365-377, 2014.

S. Chanda, R. Dave, In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview, Afr. J. Microbiol. Res, 3(13), 981‐996, 2009.

Nataraj Loganayaki, Perumal Siddhuraju, Sellamuthu Manian, Antioxidant activity and free radical scavenging capacity of phenolic extracts from Helicteres isora L. and Ceiba pentandra L, J. Food Sci. Technol. 50(4):687–695, 2013. https://dx.doi.org/10.1007%2Fs13197-011-0389-x

Mohammad Ali Ebrahimzadeh, Masoumeh Khalili, Abbas Ali Dehpour, Antioxidant activity of ethyl acetate and methanolic extracts of two marine algae, Nannochloropsis oculata and Gracilaria gracilis – an in vitro assay, Braz. J. Pharm. 54(1), 17280, 2018. DOI: 10.1590/s2175-97902018000117280

P.P. Kumar, S. Kumaravel, C. Lalitha, Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo, Afr. J. Biochem. Res. 4(7), 191–195, 2010.


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