Antioxidant potentials of successive green solvent extracts from the unexplored Ficus subincisa

Priyanka Pokhriyal, Abha Shukla, Rishi Kumar Shukla

Abstract


Introduction-Ficus subincisa Buch-Ham.Ex Sm. is considered as an endemic medicinal plant. The current research based on antioxidant potential of four different green solvent extracts obtained by the successive solvent extraction method from the unexplored F.subincisa leaves was evaluated. Various in vitro assays were performed to achieve the aim and variation in scavenging potential of the four green extracts, showed highest Total phenolic content (TPC),Total flavonoid content (TFC) and free radical scavenging potential against DPPH, ABTS,FRAP Phosphomolybdate and nitric oxide radical scavenging assay as compared to other extracts.

Method-The extraction of Ficus subincisa with diiferent green solvent namely: d-limmonene, isopropyl alcohol, ethyl lactate, and hydroalcohol.Extracts were evaluated for their antioxidant activity using different assays namely, DPPH, ABTS, and nitric oxide scavenging.The Folin-Ciocalteau and AlCl3 colorimetric method were measured the content of total phenolics and total flavonoids respectively. Reducing power was determined by phosphomolybdate and Ferric reducing antioxidant power (FRAP) methods.

 

Result-Considerable amount of phenolic and flavonoid contents were recorded in the hydroalcohol extract. Although hydroalcohol fractions exhibited good antioxidant activities, the most distinguished radical scavenging potential.Hydroalcohol sowed equivalent to standard in radical scavenging activity by DPPH.Hydroalcohol showed the higher radical scavenging activity by DPPH (92.03%), Nitric oxide (89.21%) and by ABTS (89.45%), antioxidant activity, the highest total phenols content (78.67 ± 1.07 mg GAE/g fraction), the highest total flavonoids content (195.58 ± 3.31 mg quercetin equivalent/g extract fraction). The hydroalcohol extract showed an reducing power of (93.2%) and (1373.91 ± 4.35 µM/ml Ascorbic acid equivalent) using the phosphomolybdate and FRAP assays, respectively.

 

Conclusion-Hydroalcohol shows the strongest antioxidant activity, and it can be attributed to its high content in phenolic and flavonoid compounds. It can be concluded that leaves of ficus subincisa can be used as an effective natural source of antioxidant, and as a commercial basis for the evolution of nutraceuticals

Keywords


Ficus subincisa, green solvent extracts,Total phenol content, Total flavonoid content, Free radicals, Antioxidant activity

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